Friday, March 9, 2012

Western Blot Numbers (Explained)

NEWER INFO JUNE 1,2011 BY DR. KENT HOLTORF; see his western blot explanations on numbers and LESS required to be positive than before!

*******************this explains the numbers only! more text elsewhere*******************

IgG is considered a past bacterial infection and IgM is considered a present bacteria (Not sure how accurate that is)  Looking forward to doing my IgeneX test.

On the outer surface of the Lyme bacteria are various proteins. As they have been discovered, they have been assigned letters, such as outer surface proteins A, B, and C.

The following is a brief explanation of the test results.

Again, each band is an antigen complexed (bound together) with an antibody made by the immune system, specifically for that antigen (part) of Borrelia burgdorferi.

No test for Lyme Disease is completely reliable, and results can vary by lab.  The CDC requires several Lyme-specific bands to be present for the test to be considered positive; however, many people who have Lyme do not have this many bands.  Band 23-25 is a Lyme specific band.  It is significan if it is positive.

18: An outer surface protein.

22: Possibly a variant of outer surface protein C.

23-25: Outer surface protein C (osp C).

28: An outer surface protein.

30: Possibly a variant of outer surface protein A.

31: Outer surface protein A (osp A). 34: Outer surface protein B (osp.

37: Unknown, but it is in the medical literature that it is a borrelia-associated antibody. Other labs consider it significant.

39: Unknown what this antigen is, but based on research at the National Institute of Health (NIH), other Borrelia (such as Borrelia recurrentis that causes relapsing fever), do not even have the genetics to code for the 39 kDa antigen, much less produce it. It is the most specific antibody for borreliosis of all.

41: Flagella or tail protien of all spirochetes;  this is usually the first to appear after a Bb infection and is specific for all Borrellia.  (This is how Borrelia burgdorferi moves around, by moving the flagella. Many bacteria have flagella)

This is the most common borreliosis antibody.

45: Heat shock protein. *This helps the bacteria survive fever. *The only bacteria in the world that does not have heat shock proteins is Treponema pallidum, the cause of syphilis.  Sometimes people with Lyme who have this band positive also have the co-infection Ehrlichiosis*

58: Heat shock protein.

66: Heat shock protein.

This is the second most common borrelia antibody.

73: Heat shock protein.

83: This is the DNA or genetic material of Borrelia burgdorferi. It is the same thing as the 93, based upon the medical literature. But laboratories vary in assigning significance to the 83 versus the 93.

93: The DNA or genetic material of Borrelia burgdorferi.

The committee proposed limiting the bands that could be reported in a Western Blot for diagnosis of Lyme disease. Out of a possible 25 bands, 10 specific bands were selected as being reportable.

An lgG Western Blot must have five or more of these bands: 18, 21,28, 30, 39, 41,,45, 58, 66 and 93 kDa.

An lgM Western Blot must have two or more of the following three bands: 23, 39, 41.

[b]Conspicuously absent are the most important bands, 22, 23, 25, 31, and 34, which include OSPA, OSP-B and OSP-C antigens - the three most widely accepted and recognized Bb antigens.

These antigens were the antigens chosen for human vaccine trials. In my clinical experience, if a patient has symptoms suspicious for borreliosis, and has one or more of the following bands, there is a very high probability the patient has borreliosis.

These bands are 18, 22, 23-25, 28, 30, 31, 34, 37, 39, 41, 83, and 93.

This is true regardless of whether it is IgG or IgM.. But again, there is no universal agreement on the significance of these bands. Betina Wilska, M.D. from Germany is one of the world's experts on outer surface protein A (31 kDa).

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